March 16, 2012
Identification of XMRV Infection-Associated microRNAs in Four Cell Types in Culture
Ketha V. K. Mohan1#
, Krishnakumar Devadas2#
, Shilpakala Sainath Rao1
, Indira Hewlett2
, Chintamani Atreya1*1
Section of Cell Biology, Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America, 2
Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
XMRV is a gammaretrovirus that was thought to be associated with prostate cancer (PC) and chronic fatigue syndrome (CFS) in humans until recently. The virus is culturable in various cells of human origin like the lymphocytes, NK cells, neuronal cells, and prostate cell lines. MicroRNAs (miRNA), which regulate gene expression, were so far not identified in cells infected with XMRV in culture.
Two prostate cell lines (LNCaP and DU145) and two primary cells, Peripheral Blood Lymphocytes [PBL] and Monocyte-derived Macrophages [MDM] were infected with XMRV. Total mRNA was extracted from mock- and virus-infected cells at 6, 24 and 48 hours post infection and evaluated for microRNA profile in a microarray.
MicroRNA expression profiles of XMRV-infected continuous prostate cancer cell lines differ from that of virus-infected primary cells (PBL and MDMs). miR-193a-3p and miRPlus-E1245 observed to be specific to XMRV infection in all 4 cell types. While miR-193a-3p levels were down regulated miRPlus-E1245 on the other hand exhibited varied expression profile between the 4 cell types.
The present study clearly demonstrates that cellular microRNAs are expressed during XMRV infection of human cells and this is the first report demonstrating the regulation of miR193a-3p and miRPlus-E1245 during XMRV infection in four different human cell types. Citation:
Mohan KVK, Devadas K, Sainath Rao S, Hewlett I, Atreya C (2012) Identification of XMRV Infection-Associated microRNAs in Four Cell Types in Culture. PLoS ONE 7(3): e32853. doi:10.1371/journal.pone.0032853Editor:
K.T. Jeang, National Institute of Health, United States of AmericaReceived:
October 28, 2011;
January 31, 2012; Published:
March 16, 2012
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.Funding:
SSR is a recipient of a postdoctoral fellowship at the Center for Biologics Evaluation and Research administered by the Oak Ridge Institute for Science and Education through an intra-agency agreement between the U.S. Department of Energy and the U.S. Food and Drug Administration. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Competing interests:
The authors have declared that no competing interests exist.
* E-mail: firstname.lastname@example.org
# These authors contributed equally to this work.
Full text: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032853